Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: Higher expression of LINC01354 and lower expression of miR-153-5p was observed in GC tissues and cell lines. A. LINC01354 expression in GC cancer tissue (Ct) and paracancerous tissue (Pt) was detected by qRT-PCR. B. The predicted targeting sites of LINC01354 binding to miR-153-5p were shown in database LncBase v.2. C. miR-153-5p expression was detected by qRT-PCR. D. Correlation between the expression level of LINC01354 and miR-153-5p. E, F. qRT-PCR was performed to determine the expression of LINC01354 and miR-153-5p in GC cell lines. G. The subcellular location of LINC01354 in HGC-27 and NCI-N87 cells was examined by fluorescence in situ hybridization assay. **P < 0.01, ***P < 0.001.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Fluorescence, In Situ Hybridization

Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: Abnormally high expression of CADM2 in GC tissues. A. Wild-type LINC01354 site (Site1-4 WT) or mutant-type LINC01354 (Site1-4 MUT) reporters and NC mimic or miR-153-5p were co-transfected into HEK-293T cells. Firefly/Renilla luciferase activity was measured. B. The binding site of miR-153-5p to CADM2 3’UTR was shown in the database TargetScanHuman 7.2. C. A dual-luciferase reporter assay was used to assess the relation between miR-153-5p and CADM2. D, E. CADM2 expression and its relation to the survival of GC patients were shown in the TCGA Database. F. CADM2 expression in GC cancer tissue (Ct) and paracancerous tissue (Pt) was measured by qRT-PCR. G. Association between the levels of LINC01354 and CADM2 mRNA. H. Association between the levels of miR-153-5p and CADM2 mRNA. ***P < 0.001.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Expressing, Mutagenesis, Transfection, Luciferase, Activity Assay, Binding Assay, Reporter Assay, Quantitative RT-PCR

Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: LINC01354 knockdown inhibited EMT progress by decreasing CADM2 expression. LINC01354 knockdown was performed in NCI-N87 and HGC-27 cells. A. Levels of LINC01354, miR-153-5p, and CADM2 mRNA were measured by qRT-PCR. B. Proteins of CADM2, E-cadherin, N-cadherin, and Vimentin were determined by western blotting. C, D. Proteins of CADM2, E-cadherin, and N-cadherin were assessed by immunofluorescence assay. E. Cell activity was assessed by a CCK8 assay. F. A wound-healing assay was performed to evaluate cell migration. G, H. Cell migration and invasion were detected by using a Transwell chamber. ***P < 0.001.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Activity Assay, CCK-8 Assay, Wound Healing Assay, Migration

Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: miR-153-5p overexpression attenuated the influence of LINC01354 on GC cells. LINC01354 and miR-153-5p were co-expressed in MKN-45 and SNU-1 cells. A. The levels of LINC01354, miR-153-5p, and CADM2 mRNA were measured via qRT-PCR. B. Proteins of CADM2, E-cadherin, N-cadherin, and Vimentin were determined by western blotting. C, D. Proteins of CADM2, E-cadherin, and N-cadherin were explored by immunofluorescence assay. E. A wound-healing assay was performed to assess cell migration. F, G. Cell migration and invasion were detected by using a Transwell chamber. ***P < 0.001, vs. Vector group; ###P < 0.001, vs. LINC01354 group.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Immunofluorescence, Wound Healing Assay, Migration, Plasmid Preparation

Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: CADM2 knockdown antagonized the effects of LINC01354 overexpression. LINC01354 overexpression plasmid and siRNA-targeted CADM2 were co-transfected in MKN-45 and SNU-1 cells. A. Proteins of CADM2, E-cadherin, N-cadherin, and Vimentin were determined through western blotting. B. A wound-healing assay was performed to assess cell migration. C, D. Cell migration and invasion were detected by a Transwell chamber. ***P < 0.001, vs. Vector+siNC group; ###P < 0.001, vs. LINC01354+siNC group.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Knockdown, Over Expression, Plasmid Preparation, Transfection, Western Blot, Wound Healing Assay, Migration

Journal: American Journal of Cancer Research

Article Title: LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression

doi:

Figure Lengend Snippet: CADM2 overexpression inhibited the influence of miR-153-5p on GC cells. CADM2 and miR-153-5p were co-expressed in NCI-N87 and HGC-27 cells. A. Levels of LINC01354, miR-153-5p, and CADM2 mRNA were measured by qRT-PCR. B. Proteins of CADM2, E-cadherin, N-cadherin, and Vimentin were determined by western blotting. C, D. Proteins of CADM2, E-cadherin, and N-cadherin were evaluated by immunofluorescence assay. E. A wound-healing assay was performed to assess cell migration. F, G. Cell migration and invasion were detected by a Transwell chamber. ***P < 0.001, vs. Vector group; ###P < 0.001, vs. miR-153-5p group.

Article Snippet: Small interfering RNA-specific targeting LINC01354 (5’-TGAATGTAAAATCCAAAAGCT-3’) was synthesized by RiBobio Inc. (Guangzhou, China).

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Immunofluorescence, Wound Healing Assay, Migration, Plasmid Preparation

Journal: Annals of Translational Medicine

Article Title: SOX2 amplification and chromosome 3 gain significantly impact prognosis in esophageal squamous cell carcinoma

doi: 10.21037/atm-20-1290

Figure Lengend Snippet: Gene copy number variation and SOX2 protein expression status in esophageal squamous cell carcinoma tissue assessed by fluorescence in-situ hybridization (1,000×) and immunohistochemistry (100×). (A) SOX2 disomy or low polysomy; (B) chromosome 3 gain with three or more green signals; (C) SOX2 amplification; (D) negative SOX2 expression; (E) low SOX2 expression; (F) high SOX2 expression.

Article Snippet: Briefly, a SOX2 target probe (red fluorescent signal) spanning the locus 3q26.33 and a green centromeric probe on chromosome 3 (Empire Genomics, New York, USA) were selected for hybridization.

Techniques: Expressing, Fluorescence, In Situ Hybridization, Immunohistochemistry, Amplification

Journal: Annals of Translational Medicine

Article Title: SOX2 amplification and chromosome 3 gain significantly impact prognosis in esophageal squamous cell carcinoma

doi: 10.21037/atm-20-1290

Figure Lengend Snippet: Associations between SOX2 status and patient characteristics

Article Snippet: Briefly, a SOX2 target probe (red fluorescent signal) spanning the locus 3q26.33 and a green centromeric probe on chromosome 3 (Empire Genomics, New York, USA) were selected for hybridization.

Techniques: Expressing

Journal: Annals of Translational Medicine

Article Title: SOX2 amplification and chromosome 3 gain significantly impact prognosis in esophageal squamous cell carcinoma

doi: 10.21037/atm-20-1290

Figure Lengend Snippet: Kaplan-Meier survival curves for disease-free survival (DFS, A) and overall survival (OS, B). A significantly shorter DFS or OS was observed in the SOX2 amplification group, while a significantly longer DFS or OS was observed in the chromosome 3 gain group, compared with the group with low polysomy or disomy.

Article Snippet: Briefly, a SOX2 target probe (red fluorescent signal) spanning the locus 3q26.33 and a green centromeric probe on chromosome 3 (Empire Genomics, New York, USA) were selected for hybridization.

Techniques: Amplification

Journal: Annals of Translational Medicine

Article Title: SOX2 amplification and chromosome 3 gain significantly impact prognosis in esophageal squamous cell carcinoma

doi: 10.21037/atm-20-1290

Figure Lengend Snippet: Univariate and multivariate analysis for disease-free survival (DFS) and overall survival (OS)

Article Snippet: Briefly, a SOX2 target probe (red fluorescent signal) spanning the locus 3q26.33 and a green centromeric probe on chromosome 3 (Empire Genomics, New York, USA) were selected for hybridization.

Techniques: Adjuvant, Amplification, Expressing